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Israeli forces shelled a house in the Gaza Strip which they had moved around 110 Palestinians into 24 hours earlier, the UN quotes witnesses as saying.
The UN Office for the Coordination of Humanitarian Affairs (OCHA) called it "one of the gravest incidents" since the beginning of the offensive.
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The ICRC on Thursday accused Israel of failing to fulfil its duty to help wounded civilians in Gaza.
"In Gaza, there is a severe protection of civilians crisis. There is no safe haven, no safe space, for all the civilians, particularly children," Ms Pacheco told the BBC.
"Since the ground operation, the number of children killed has risen by 250%."
An estimated 770 Palestinians and 14 Israelis have died in nearly two weeks of Israel's air and ground offensive against the Palestinian militant group Hamas.
The UN Security Council has called for an immediate ceasefire and the withdrawal of Israeli troops from Gaza.
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