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midnight armadillo Donating Member (1000+ posts) Send PM | Profile | Ignore Tue Jan-11-05 09:38 AM
Original message
Hey DU scientists - spectroscopy question
Are there any applications of UV spectroscopy in biology/biophysics/etc? I can't for the life of me think of any - my experience is all in the visible, NIR and mid-IR.

I'm asking because my company is developing a spectrometer and we're eyeing various applications for it in order to chase fed funding. Our university collaborators are in the biology/medicine/biophysics field. I will ask them when they're back from winter break :-)
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MrMonk Donating Member (1000+ posts) Send PM | Profile | Ignore Tue Jan-11-05 09:47 AM
Response to Original message
1. The use of UV spectroscopy goes back over 50 years ...
and is still widely used. I used to use it to identify aromatic compounds and, later, to monitor progress of enzymatic reactions.

Google " "UV Spectroscopy" (biochemical OR biophysical) " and you'll
find plenty of references.
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kitkatrose Donating Member (1000+ posts) Send PM | Profile | Ignore Tue Jan-11-05 09:48 AM
Response to Original message
2. I remember
UV being used to identify conjugation in compounds (alternating single and double bonds). So I guess you could use it for compound identification, but I haven't a clue beyond that. :shrug:
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Donkeyboy75 Donating Member (1000+ posts) Send PM | Profile | Ignore Wed Jan-12-05 01:27 PM
Response to Original message
3. It's often used to monitor protein expression in cells.
The wavelength around 254 nm is used.
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slutticus Donating Member (1000+ posts) Send PM | Profile | Ignore Wed Jan-12-05 03:15 PM
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4. Absolutely!
I use it all the time. Proteins and nucleic acids have large extinction coeffs at 280nm and 260nm. I use it to measure concentrations. I'm sure there are many other applications.

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