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Sun Dec 2, 2012, 06:20 AM

DNA imaged with electron microscope for the first time



It's the most famous corkscrew in history. Now an electron microscope has captured the famous Watson-Crick double helix in all its glory, by imaging threads of DNA resting on a silicon bed of nails. The technique will let researchers see how proteins, RNA and other biomolecules interact with DNA.

The structure of DNA was originally discovered using X-ray crystallography. This involves X-rays scattering off atoms in crystallised arrays of DNA to form a complex pattern of dots on photographic film. Interpreting the images requires complex mathematics to figure out what crystal structure could give rise to the observed patterns.

The new images are much more obvious, as they are a direct picture of the DNA strands, albeit seen with electrons rather than X-ray photons. The trick used by Enzo di Fabrizio at the University of Genoa, Italy, and his team was to snag DNA threads out of a dilute solution and lay them on a bed of nanoscopic silicon pillars.

The team developed a pattern of pillars that is extremely water-repellent, causing the moisture to evaporate quickly and leave behind strands of DNA stretched out and ready to view. The team also drilled tiny holes in the base of the nanopillar bed, through which they shone beams of electrons to make their high-resolution images. The results reveal the corkscrew thread of the DNA double helix, clearly visible. With this technique, researchers should be able to see how single molecules of DNA interact with other biomolecules.


More at Link: http://www.newscientist.com/article/dn22545-dna-imaged-with-electron-microscope-for-the-first-time.html

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Reply DNA imaged with electron microscope for the first time (Original post)
littlemissmartypants Dec 2012 OP
eppur_se_muova Dec 2012 #1

Response to littlemissmartypants (Original post)

Sun Dec 2, 2012, 08:31 AM

1. First time ? Hardly. Maybe at the best resolution so far.

http://en.wikipedia.org/wiki/Transmission_electron_microscopy_DNA_sequencing



Google "DNA electron microscope", there are lots of DNA images out there. When I first entered grad school in 1981 this was already being discussed in Molecular Biology classes for undergrads. Why the journo would have thought this is the first such image, I don't know -- perhaps it's the first one without metal coating (normally used to enhance scattering)? Not enough detail in this short article to tell.

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