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Sat Jan 26, 2013, 10:15 AM

Prevalence and persistence of male DNA identified in mixed saliva samples after intense kissing

Abstract

Identification of foreign biological material by genetic profiling is widely used in forensic DNA testing in different cases of sexual violence, sexual abuse or sexual harassment. In all these kinds of sexual assaults, the perpetrator could constrain the victim to kissing. The value of the victim's saliva taken after such an assault has not been investigated in the past with currently widely used molecular methods of extremely high sensitivity (e.g. qPCR) and specificity (e.g. multiplex Y-STR PCR). In our study, 12 voluntary pairs were tested at various intervals after intense kissing and saliva samples were taken from the women to assess the presence of male DNA. Sensitivity-focused assays based on the SRY (single-copy gene) and DYS (multi-copy gene) sequence motifs confirmed the presence of male DNA in female saliva after 10 and even 60min after kissing, respectively. For specificity, standard multiplex Y-STR PCR profiling was performed and male DNA was found in female saliva samples, as the entire Y-STR profile, even after 30min in one sample. Our study confirms that foreign DNA tends to persist for a restricted period of time in the victim's mouth, can be isolated from saliva after prompt collection and can be used as a valuable source of evidence.


http://www.ncbi.nlm.nih.gov/pubmed/22917815

Emphasis added.

By kissing someone, you are providing them with a sample of your DNA. So only kiss someone you absolutely trust.

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Reply Prevalence and persistence of male DNA identified in mixed saliva samples after intense kissing (Original post)
FarCenter Jan 2013 OP
DURHAM D Jan 2013 #1
boston bean Jan 2013 #4
mzteris Jan 2013 #2
MNBrewer Jan 2013 #5
randome Jan 2013 #3

Response to FarCenter (Original post)

Sat Jan 26, 2013, 10:22 AM

1. Huh? Unrec for last sentence.

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Response to DURHAM D (Reply #1)

Sat Jan 26, 2013, 11:16 AM

4. agreed. nt

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Response to FarCenter (Original post)

Sat Jan 26, 2013, 11:06 AM

2. Wasn't this in

Gattica?

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Response to mzteris (Reply #2)

Sat Jan 26, 2013, 11:21 AM

5. Yup!

Love that film.

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Response to FarCenter (Original post)

Sat Jan 26, 2013, 11:10 AM

3. I would guess that most genetic sampling occurs more than 60 min after an alleged crime.

Still interesting, though.

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